Cell migration assays

Cell migration assays

Easily quantify migratory cells without manual cell counting

 

2D Gap-closure Assays

It creates a defined area on a two-dimensional surface, such as the bottom of a cell culture plate well, across which migratory cells may move.

  • Quantitatively or qualitatively measurement
  • Any cell type migration 
  • At the endpoint or in real time 
  • By microscopic detection

Measure differences in migration rates between


 Boyden chamber Assays

It consists of a cell culture insert nested in the well of a cell culture plate. The bottom of the insert contains a membrane with a specific pore size. Cells are seeded in the top of the insert and move through the membrane pores to the underside

  • Quantitatively measurement
  • Numerous cell types migration 
  • At the endpoint 
  • By colorimetric or fluorescence detection

Measure effect of chemoattractant on migration rates 

 
 
Methods Assays Cell types Insert coating Format Cat #
2D Gap-closure Cell migration assay Any
None
24 Assays CBA-125
Collagen I
24 Assays CBA-125-COL
ECM Array
24 Assays CBA-125-ECM
Fibronectin
24 Assays CBA-125-FN
Laminin
24 Assays CBA-125-LN
None 96 Assays CBA-126
None
384 Assays CBA-127
Boyden Chamber Chemotaxis Fibroblasts,
Endothelial Cells,
Epithelial Cells,
Tumor Cells
(
8 μm)
None
24-Well CBA-100
None 24-Well CBA-101
None 96-Well CBA-106
Monocytes,
Macrophages
(
5 μm)
None 24-Well CBA-102
None 96-Well CBA-105
Neutrophils, other
Leukocytes
(
3 μm)
None 24-Well CBA-103
None 96-Well CBA-104
Astrocytes,
Slow Moving Cells
(
12 μm)
None 24-Well CBA-107
None 24-Well CBA-108
Haptotaxis
Fibroblasts,
Endothelial Cells,
Epithelial Cells
(8 μm)
Collagen I
(bottom)
24-Well CBA-100-COL
Collagen I
(bottom)
24-Well CBA-101-COL
Fibronectin
(bottom)
24-Well CBA-100-FN
Fibronectin
(bottom)
24-Well CBA-101-FN
Transmigration
Leukocytes
(
3 μm)
None
24-Well CBA-212
Tumor Cells
 (
8 μm)
None 24-Well CBA-216

You can also monitor cellular adhesion, invasion and wound healing