SARS-CoV-2 - RT-qPCR for research and development
The COVID-19 RT-qPCR assay is based on the detection of SARS-CoV-2 RNA using a real-time RT-PCR technique with probes and primers specific to certain SARS-CoV-2 genes.
The coronavirus real-time RT-PCR nucleic acid detection kits (SARS-CoV-2) are based on the PCR method which uses a fluorescent probe and a specific primer to detect two or three specific regions within the genome of the new coronavirus (SARS-CoV-2). Typically, these are the Orf1ab, N and E genes. This detection can be done separately for each gene or in multiplex (all genes in the same reaction).
The test is performed on viral RNA extracted from nasopharyngeal swabs or salive, usually or more rarely from serum, plasma or whole blood samples from patients who may be infected with CoV-2-SARS. We also offer kits for RNA preservation in saliva samples and kits for extraction and purification of viral RNA.
The QuantiVirus Real-Time PCR Coronavirus Test kit is part of the list of official kits for the diagnosis of CoV-2-SARS in France.
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